MASCOT Search Results

Search metadata
User : Ricarda
E-mail : Haberlea_dry
MS data file : Haberlea_dry.mgf
Database : Tair10 20150119 (35,386 sequences; 14,482,855 residues)
Timestamp : 26 Feb 2020 at 02:54:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Variable modifications : Carbamidomethyl (C), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm (# 13C = 1)
Fragment mass tolerance : ± 0.5 Da
Max missed cleavages : 1
Instrument type : ESI-QUAD-TOF
Number of queries : 4,939

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.

There are 60 peptide matches above identity threshold and 102 matches above homology threshold for 4,939 queries. Histogram score range is (0, 78). On average, individual ions scores > 27 (beyond green shading) indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Modification statistics
Modification Site Above thr.
Oxidation M 8
Legend
Dupes Expect Rank U 1 2 Peptide
0.037 Expand2 GAYSLSLR significant
9 Expand1 GFFLFVEGGR top ranking
6.4e-05 Expand1 GSSIFGLAPGK significant and top ranking
1.3e-06 Expand1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-07 Expand1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-05 Expand1 U GSSIFGLAPGK unique
Expand2 5.7e-05 Expand1 LNTLETEEWFFK peptide has two duplicates
0.18 Expand1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (Expand) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (Collapse) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Taxonomy

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Protein families 1–10 (out of 56)


Page: 1 2 3 4 5 6 Next 

Expand1

Accession Score Description
1 AT1G78900.1 148 | Symbols: VHA-A | vacuolar ATP synthase subunit A | chr1:29660463-29664575 FORWARD LENGTH=623

Expand2

Accession Score Description
1 ATCG00680.1 104 | Symbols: PSBB | photosystem II reaction center protein B | chrC:72371-73897 FORWARD LENGTH=508

Expand3

Accession Score Description
1 ATCG00120.1 93 | Symbols: ATPA | ATP synthase subunit alpha | chrC:9938-11461 REVERSE LENGTH=507

Expand4

Accession Score Description
1 AT3G52990.1 93 | Symbols: | Pyruvate kinase family protein | chr3:19649046-19652237 FORWARD LENGTH=527

Expand5

Accession Score Description
Family member distances as a dendrogram 1 ATCG00480.1 83 | Symbols: ATPB, PB | ATP synthase subunit beta | chrC:52660-54156 REVERSE LENGTH=498
2 AT5G08670.1 69 | Symbols: | ATP synthase alpha/beta family protein | chr5:2818395-2821149 REVERSE LENGTH=556

Expand6

Accession Score Description
1 AT1G20260.1 76 | Symbols: | ATPase, V1 complex, subunit B protein | chr1:7016971-7020290 FORWARD LENGTH=487

Expand7

Accession Score Description
1 AT3G09790.1 72 | Symbols: UBQ8 | ubiquitin 8 | chr3:3004111-3006006 REVERSE LENGTH=631

Expand8

Accession Score Description
1 AT4G04640.1 70 | Symbols: ATPC1 | ATPase, F1 complex, gamma subunit protein | chr4:2350761-2351882 REVERSE LENGTH=373

Expand9

Accession Score Description
1 AT2G27030.1 65 | Symbols: CAM5, ACAM-2 | calmodulin 5 | chr2:11532069-11533060 FORWARD LENGTH=149

Expand10

Accession Score Description
1 AT1G79750.1 59 | Symbols: ATNADP-ME4, NADP-ME4 | NADP-malic enzyme 4 | chr1:30007655-30011179 REVERSE LENGTH=646

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